تأثیر پرتوتابی لیزر بر روی صفات جوانه‌زنی جو در محیط‌های شور در مرحله‌ی جوانه‌زنی

نوع مقاله: مقاله فنی

نویسندگان

1 گروه علوم زراعی و اصلاح نباتات، پردیس ابوریحان دانشگاه تهران

2 پژوهشکده‌ بیوتکنولوژی کشاورزی ایران

چکیده

به منظور بررسی اثر پرتوتابی لیزر در تحمل به شوری گیاه جو در مرحله­ی ­­جوانه­‌زنی، در گذشته در کشور به صورت آزمایش فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار انجام شده است. عوامل مورد بررسی شامل چهار ژنوتیپ جو (ریحان، افضل، فجر 30 و نصرت) دو سطح پرتوتابی لیزر (بدون تابش و با تابش لیزر به طول موج nm 780 به مدتmin 15) و 5 سطح شوری (°، 80، 160، 240 و
mM 320 از کلرید سدیم) بودند. در این آزمایش در صفات جوانه­‌زنی (طول ساقه‌­چه، طول ریشه­‌چه، طول کلئوپتیل، تعداد ریشه­‌های بذری، درصد جوانه‌­زنی، سرعت جوانه‌­زنی، میانگین مدت جوانه‌­زنی، وزن خشک گیاه‌چه و بنیه‌­ی بذر)، در سطوح شوری و پرتوتابی در بین ارقام مورد بررسی تفاوت معنی­‌داری مشاهده شد. با افزایش سطح شوری، از میزان مهم‌­ترین صفت جوانه‌­زنی که شاخص بنیه­‌ی بذر بود، در ارقام مورد مطالعه کم شد. پرتوتابی لیزر در این صفت به ترتیب باعث افزایش 39، 63 و 46% در ارقام افضل، ریحان و فجر 30 شد. به طور کلی، با توجه به تأثیرات مثبت لیزر بر فاکتورهای مورد بررسی چنین به نظر می­‌آید که پرتوتابی لیزر می‌­تواند تیماری مؤثر برای بالا بردن میزان تحمل گیاه نسبت به تنش شوری باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effect of Laser Irradiation on Germination of Barley in Saline Condition at the Germination Stage

نویسندگان [English]

  • S. A sadat Noori 1
  • F Amini 1
  • B Foghi 1
  • Z Hamidi 1
  • N. A Khoshkhalgh Sima 2
چکیده [English]

To evaluate the effect of laser irradiation on the salinity tolerance in barley (hordeum vulgare L.) in the germination phase and seedling, a three- replicate experiment with CRD in a factorial arrangement experimental type has been previously conducted in Iran. Experimental factors were four barley genotypes (Reihan, Afzal, Fajr30 and Nosrat), two radiation levels (no laser and laser irradiation at 780 nm wavelength for 15 minutes) and five levels of salinity (0, 80, 160, 240 and 320 mM of sodium chloride). In this research, in the germination parameters (root length, shoot length, coleoptile length, root number, germination percentage, mean germination time, dry weight of seedling, seed vigor, and germination rate), all genotypes were significantly affected by increasing the salinity and laser irradiation (P<0.01). There was a reduction in the seed vigor (the most important germination parameter) by increasing the salinity level. The laser pre-treatment caused an increase of 39, 63, 46 and 100 percent in Afzal, Reihan, fajr 30 and Nosrat genotypes, respectively. The overall result showed a positive effect of the laser irradiation on the investigated traits.

کلیدواژه‌ها [English]

  • Barley
  • Germination
  • Laser Irradiation
  • Salinity Stress
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