غربال‌گری عوامل مؤثر بر جذب‌زیستی اورانیم از محلول‌های آبی با استفاده از باکتری Pseudomonas Putida تثبیت ‌شده در کیتوزان

نوع مقاله: مقاله پژوهشی

نویسندگان

پژوهشکده‌ی مواد و سوخت هسته‌ای، پژوهشگاه علوم و فنون هسته‌ای، سازمان انرژی اتمی ایران

چکیده

جداسازی فلزهای سنگین از پساب صنعتی، معضل زیست‌­محیطی جدی به ویژه در کشورهای در حال توسعه است. از میان فناوری­‌های آمایشی متعدد، به نظر می‌­رسد که جذب‌­زیستی گزینه­‌ی مناسبی باشد. ­جاذب­‌های زیستی پایه میکروبی برای حذف فلزهای سنگین از محلول­‌های آبی، مؤثر و کاربردی­‌اند. در مطالعه­‌ی حاضر، توانایی باکتری Pseudomonas putida تثبیت ­شده بر روی کیتوزان برای جذب‌­زیستی اورانیم (VI) از محلول‌­های آبی بررسی شد. غربال­‌گری عوامل مؤثر بر فرایند جذب­‌زیستی به کمک طراحی پلکت- برمن صورت گرفت. نتایج تجربی نشان داد که مقدار جاذب‌­زیستی، غلظت اولیه اورانیم، اندازه­‌ی ذرات جاذب­‌زیستی، درصد وزنی باکتری در جاذب‌­زیستی و pH، به­ ترتیب بیش­ترین تأثیر را بر میزان جذب دارند، در حالی­که دما یک پارامتر غیرمؤثر تشخیص داده شد. بیشینه‌­ی ظرفیت جذب­‌زیستی عملی برای جاذب­‌زیستی حاوی 15% وزنی باکتری و معادلmg/g  536.08 به دست آمد. ایزوترم‌­های جذب­‌زیستی با استفاده از مدل­‌های لانگمویر، فرندلیچ و دابینین- رادوشکویچ تحلیل شد. برای جاذب‌­زیستی، مدل لانگمویر (0.983=R2) بهترین برازش را با داده­‌های تعادلی از خود نشان داد و بیشینه ظرفیت به دست آمده از این مدل برای این جاذب‌­زیستی 588.23 و برای کیتوزان خالص mg/g 454.54 بود. در نتیجه، مطالعه‌­ی حاضر نشان داد که جاذب‌­زیستی تهیه شده می­‌تواند موردی مناسب برای جذب‌­زیستی اورانیم باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Screening of Significant Factors in Uranium Biosorption from Aqueous Solutions Using Pseudomonas Putida Immobilized on Chitosan

نویسندگان [English]

  • H Sohbatzadeh Lonbar
  • A Keshtkar
  • J Safdari
  • F Fatemi Ardestani
  • M Ghasemi Torkabadi
چکیده [English]

Heavy metal sequestration from industrial wastewater is a serious environmental problem especially in developing countries. Among various treatment technologies, it seems that biosoprtion is a promising alternative method. Microbial–based biosorbents are effective and applicable for heavy metals removal from aqueous solutions. The present study investigated the ability of Pseudomonas putida immobilized on chitosan to adsorb uranium (VI) from the aqueous solution. The biosorption process factors were screened using Plackett–Burman design. The results showed that the biosorbent dosage, initial concentration, biosorbent particle size, bacteria wt.% in the biosorbent and pH were the most significant parameters, respectively while temperature was the only insignificant parameter in the biosorption process. Maximum practical biosorption capacity was 536.08 mg g–1 obtained from 15 wt.% of the bacterial cells immobilized on chitosan. Biosorption equilibrium isotherms were analyzed by Langmuir, Freundlich and Dubinin–Radushkevhch models. For Pseudomonas putida immobilized on chitosan, the Langmuir isotherm model (R2=0.983) was proved to fit the equilibrium data best with the maximum capacities of 588.23 and 454.54 mg g–1 for the biosorbent and pure chitosan, respectively. In conclusion, the present study indicated that the prepared composite biosorbent could be a suitable candidate for uranium (VI) biosorption.

کلیدواژه‌ها [English]

  • Biosorption
  • Uranium(VI)
  • Bacterium
  • Pseudomonas Putida
  • Chitosan
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