ایجاد جهش در صفات مرتبط با خورجین در ارقام کلزا با استفاده از اشعه‌ی گاما

نوع مقاله: مقاله پژوهشی

نویسنده

گروه زراعت و اصلاح نباتات، دانشکده‌ی علوم کشاورزی،دانشگاه شاهد

چکیده

بذور ارقام کلزای ساری گل و RGS003 با اشعه­ی گاما در دزهای °، 800، 1000 و Gy1200 تیمار شدند. در نسل 2M، بذر هر بوته در یک ردیف جداگانه کشت شد و کلیه­‌ی بوته­‌های هر لاین در طول فصل زراعی بررسی شدند. در این نسل تعداد 18 لاین موتان از رقم ساری گل و 12 لاین موتان از رقم RGS003 با توجه به تغییرات مشاهده شده در صفات زراعی مانند طول خورجین، تعداد خورجین و زمان گل­دهی به دست آمد. بین دزهای اشعه‌­ی گاما از نظر عقیمی دانه­‌ی گرده، تفاوت معنی­‌دار در سطح احتمال 1% مشاهده شد، ولی این تفاوت برای عقیمی بذر معنی­‌دار نبوده است. حداکثر عقیمی دانه‌­ی گرده در دز Gy800 مشاهده شد. بیش­تر موتان­‌های به دست آمده در ارقام RGS003 و ساری گل به ترتیب در دزهای 1200 و Gy800 به دست آمدند. در نسل 3M، موتان­‌های به دست آمده در هر دو رقم کلزا برای صفاتی مانند تعداد خورجین در ساقه­‌ی اصلی، تعداد خورجین در بوته، طول خورجین، تعداد دانه در خورجین، وزن هزار دانه و طول دوره­‌ی گل­دهی تغییرات داشتند.

کلیدواژه‌ها


عنوان مقاله [English]

Induction of Mutation for Traits Related to Pod in Rapeseed (Brassica Napus L.) Cultivars Using Gamma Rays

نویسنده [English]

  • M. H Fotokian
چکیده [English]

The grains of cultivars Rapeseed Sarigol and RGS003 were irradiated with gamma rays at doses 0, 800, 1000 and 1200 Gary (GY). For M2 generation, the grain of each plant was cultivated in a separate row and all plants were individually investigated during cultivation. According to the observed changes in agronomic traits such as pod length, the number of pods and flowering during M2 generation, 18 and 12 candidate mutant lines were obtained from Sarigol and RGSOO3, respectively. The differences among gamma rays doses were not statistically significant for seed sterility, while they were significant for pollen sterility at the level of 1%. Maximum pollen sterility was observed in 800 GY. Induced mutations in RGS003 and Sariglo cultivars were mostly obtained in 1200 and 800 GY, respectively. The M3 mutant lines in both Rapeseed cultivars contained a variation on traits such as the number of pod per the main stem, the number of pod per plant, pod length, the number of grain per pod, grain weight and flowering duration.

کلیدواژه‌ها [English]

  • Gamma Rays
  • Induced Mutation
  • Rapeseed (Brassica Napus L.)
  • Pod Length
  • Mutant

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