عنوان مقاله [English]
نویسندگان [English]چکیده [English]
[55Co] bleomycin has been widely used in oncological studies. Due to relative short half life of cobalt-55, optimization and feasibility studies for determination of the best labeling conditions is preferably performed using cobalt-57. In this study, the optimization of complexation conditions for time, temperature and ligand concentration were performed. The best labeling yield was obtained at room temperature in pH of 4-7, using 0.4 mCi of 57CoCl2 with 0.3mg of BLM in 30 minutes. The final radiopharmaceutical solution underwent common quality control tests, validating our future studies for animal scanning studies using [55Co]bleomycin complex.
10.D.Y. Hou, H. Hoch, G.S. Johnston, K.C. Tsou, R.J. Farkas, E.E. Miller, "Stability of 111In-bleomycin in vivo-properties compared with 57Co-bleomycin," Eur. J. Nucl. Med., 8(12),535-540 (1983).
11.J. Bier, E. Loer, J. Franke, "Kinetics of 57Co-bleomycin in sheep after intra-arterial injection in the head and neck region," Recent. Results. Cancer. Res., 86, 141-151 (1983).
12.J. Belehradek and L.M. Mir, "Stable [57Co]-bleomycin complex with a very high specific radioactivity for use at very low concentrations," Biochem. Biophys. Res. Commun, 173(1),259-264 (1990).
13.A.R.Jalilian, P.Rowshanfarzad, M.Sabet, A.Novinrooz, G.Raisali ,"Preparation of [66Ga]Bleomycin Complex as a Possible PET Radiopharmaceutical," J. Radioanal. Nucl. Chem. 264(3), 617-621 (2005).
14.A.R. Jalilian, B. Fateh, M. Ghergherehchi, A. Karimian, M. Matlloobi, S. Moradkhani, M. Kamalidehghan, "Preparation, distribution, stability and tumor imaging properties of [62Zn]Bleomycin in normal and tumor-bearing mice,"Iran.J.Radiat.Res., 1(1), 37-44 (2003).
15. A.R. Jalilian, P. Rowshanfarzad, A. Rahiminejad-Kisomi, S. Moradkhani, F. Motamedi, "Preparation, biodistribution and stability of [65Zn]bleomycin complex," DARU, 12(3), 115-122 (2004).