بررسی دنباله DNA با استفاده از تکنیک SCGE در نمونه های مرغ و میگو پرتودیده

نوع مقاله: مقاله پژوهشی

نویسندگان

بخش پرتودهی مواد غذایی، مرکز تابش گاما، سازمان انرژی اتمی ایران، تهرا ن صندوق پستی 3486 –11365

چکیده

DNA  سلولهای مواد غذایی بوسیله پرتو گاما شکسته شده که این شکستگی از طریق تکنیک حساسی به نام  الکتروفورز یک سلول منفرد شناسایی میگردد.  برای اثبات این منظور نمونه های مرغ و میگو با استفاده از کبالت 60  پرتو دیده و دزهای بکار رفته برای نمونه مرغ 2، 5 و 7 کیلوگری و برای نمونه میگو 3 و 7 کیلوگری بوده است. سپس نمونه های پرتودیده با پرتوندیده (کنترل) مقایسه شدند. بعلاوه اثرات طول مدت نگهداری و دما  صرفاً بر روی  نمونه ها ی مرغ مورد بررسی قرارگرفت. نتایج بدست آمده از آزمایشات مکرر نشان داد که دردزهای  متفاوت (حتی در دزهای پایین) تشخیص نمونه های پرتودیده ازکنترل  به سهولت امکان پذیر است.     

کلیدواژه‌ها


عنوان مقاله [English]

DETECTION OF DNA COMET BY USING OF SCGE FOR IRRADIATED POULTRY AND SHRIMP

نویسندگان [English]

  • R Rajaie
  • S.L Hosseini
چکیده [English]

DNA in food will sustain damage by gamma radiation that this damage was detected by a sensetive technique called single cell gel electrophoresis. For this purpose poultry and shrimp samples were irradiated by 60Co gamma radiation. The radiation doses for poultry were 2, 5 and 7 kGy and for shrimp were 3 and 7 kGy. Then, irradiated samples were compared with unirradiated ones (control). In addition, the effects of shelf-life and temperature were considered on the same samples. We found that with different doses (even at low dose( identification of irradiated from  unirradiated samples were easily possible.

کلیدواژه‌ها [English]

  • poultry
  • Shrimp
  • Irradiation
  • single cell gel electrophoresis
  • DNA comet assay
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