عنوان مقاله [English]
نویسندگان [English]چکیده [English]
Trace elements play an important role in the biophysiology of cells by affecting their growth and contributions to various biological processes such as wound healing. Determination of toxic trace elements in biological fluids is an important subject of interest for toxicological purposes. Increasing the concentration of these elements in the blood levels, cause serious diseases in patients. Recently instrumental analysis procedures such as atomic absorption spectrometry have been used in clinical measurements for determination of many toxic trace elements in the biological samples. In this paper we are reporting the study of various methods of blood and serum samples preparation for determining the toxic trace elements of Arsenic and Chromium. The measurement of this elements performed by using electrothermal atomic absorption spectrometry. The best and reliable results for Chromium analysis was achived by injection of diluted serum samples, where the samples were diluted with HCl 0.1N. In Arsenic analysis, the best results obtained by extraction of blood with aqueous solution of TCA. For determining all of these elements the RSD% was less than 5%.
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